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PBjelly2 failes at output step

Christian
2017-01-18
2017-01-18
  • Christian

    Christian - 2017-01-18

    Hi,

    I'm running PBjelly version 15.8.24. Collection.py run for 5 days (630k assembly folders) when it finally crashed while writing the final contigs. These are the last few lines of output.err:

    2017-01-17 23:23:57,350 [DEBUG] Getting fill sequence for ref0161811e5_ref0980136e3
    2017-01-17 23:23:57,350 [DEBUG] fill span
    2017-01-17 23:23:57,350 [DEBUG] Moving from ref0980136e3 to ref0980136.1e5 (p=1)
    2017-01-17 23:23:57,350 [DEBUG] contig
    2017-01-17 23:23:57,350 [DEBUG] who? ref0980136.1e5 ref0980136e3
    2017-01-17 23:23:57,350 [DEBUG] Grabbing contig between nodes ref0980136.1e5 & ref0980136e3 - [trim 0, 0]
    2017-01-17 23:23:57,350 [DEBUG] contig 919 to 7804
    2017-01-17 23:23:57,350 [DEBUG] trimming 0 and 0
    2017-01-17 23:23:57,351 [DEBUG] Moving from ref0980136.1e5 to ref0980136.0e3 (p=1)
    2017-01-17 23:23:57,501 [DEBUG] improved gap
    2017-01-17 23:23:57,501 [DEBUG] Getting fill sequence for ref0980136.0e3_ref0980136.1e5
    2017-01-17 23:23:57,501 [DEBUG] No predicted gap size
    Traceback (most recent call last):
    File "/ctx/software/PBSuite_15.8.24/bin/Collection.py", line 963, in <module>
    c.run()
    File "/ctx/software/PBSuite_15.8.24/bin/Collection.py", line 923, in run
    self.outputContigs()
    File "/ctx/software/PBSuite_15.8.24/bin/Collection.py", line 817, in outputContigs
    seq.reverseCompliment()
    AttributeError: 'NoneType' object has no attribute 'reverseCompliment'</module>

    Any idea want went wrong?
    Debugging will probably be a huge pain due to the runtime of the output stage. Is it possible to write some kind of temporary files that stores intermediate results from this stage?

    Thanks,
    Chris

     
  • Adam English

    Adam English - 2017-01-27

    Could you look in the folder for the assembly/ref0980136.0e3_ref0980136.1e5 and see what's there? Should maybe be some logs or sequence files or jsons. Something about that particular assembly is breaking the pipeline.

     
  • Urmi Trivedi

    Urmi Trivedi - 2017-05-16

    Hello Chris and Adam,
    I am facing same error.

    2017-05-09 11:18:44,904 [DEBUG] Getting fill sequence for ref0004924.2e3_ref0004924.3e5
    2017-05-09 11:18:44,904 [DEBUG] No predicted gap size
    Traceback (most recent call last):
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 963, in <module>
        c.run()
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 923, in run
        self.outputContigs()
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 818, in outputContigs
        liftTracker.append((name, strand, len(seq.seq)))
    AttributeError: 'NoneType' object has no attribute 'seq'
    

    It will be very helpful if you could share your ideas to fix this.
    Many thanks

     
    • Adam English

      Adam English - 2017-06-01

      Hello Urmi,

      Could you also look in the folder for the assembly/
      ref0004924.2e3_ref0004924.3e5 and see what's there? Should maybe be some
      logs or sequence files or jsons. Something about that particular assembly
      is breaking the pipeline.

      On Tue, May 16, 2017 at 1:46 AM, Urmi Trivedi urmi208@users.sf.net wrote:

      Hello Chris and Adam,
      I am facing same error.

      2017-05-09 11:18:44,904 [DEBUG] Getting fill sequence for ref0004924.2e3_ref0004924.3e5
      2017-05-09 11:18:44,904 [DEBUG] No predicted gap size
      Traceback (most recent call last):
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 963, in <module>
      c.run()
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 923, in run
      self.outputContigs()
      File "/lustre/software/PBSuite_15.8.24/bin/Collection.py", line 818, in outputContigs
      liftTracker.append((name, strand, len(seq.seq)))
      AttributeError: 'NoneType' object has no attribute 'seq'</module>

      It will be very helpful if you could share your ideas to fix this.
      Many thanks


      PBjelly2 failes at output step
      https://sourceforge.net/p/pb-jelly/discussion/pbjtiks/thread/ca29e9d2/?limit=25#cc3a


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